domestic rabbit monoclonal (E61) reactivity: human conjugate: HRP application: western blot, immunohistochemistry - paraffin section

2020-01-10 · Background Increasing evidence has suggested that microRNAs (miRNAs) act as key post-transcriptional regulators in tumor progression. Previous studies have confirmed that miR-17-5p functions as an oncogene in multiple cancers and contributes to tumor progression. However, the role and biological functions of miR-17-5p in the development of laryngeal squamous cell carcinoma (LSCC) still remain

Anti-rabbit Alexa Fluor 488, Life technologies  Antikropparna som användes för western blotting var följande: GFP (Abcam, ab290 DsRed (MBL, PM005, 1: 1000) och aktiverad-caspase3 (Abcam, ab13847,  BAK (D4E4, #12105), and Hsp60 (#4870); from Abcam: active caspase 3 (#​ab13847), BCL-xL (#ab32370, used at 1/500 dilution), BCL-2 (#ab18210, used at​  Primary antibodies used were as follows: anti-FAP (ab53066, Abcam; 1:500), anti​-CD31 (ab9498, Abcam; 1:1, 000), anti-active Caspase 3 (ab13847; Abcam,  UK), kanin-anti-aktivt caspase-3 (ab13847, 1: 200; Abcam), mus-anti-a-tubulin (klon B5.1.2, 1: 2000; Sigma-Aldrich), get-anti-RanBP1 (C-19) (sc-1160, 1:25;  ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), Bach2-antikropp (ab83364, Abcam) och normal mus-IgG-kontroll användes för  som användes omfattade kanin anti-LC3-antikropp (4108, Cell Signaling, USA)​, kanin-antiaktiva caspase-3 polyklonala antikroppar (ab13847, Abcam Co. ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN.

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Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam. 781 publications reference this Antibody. We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.

primary anti-Rad51 (1:500; Abcam, ab109107) antibody added overnight. Rabbit anti-active caspase 3 antibodies, Abcam, ab13847. Normal goat serum, Vector Laboratories, S-1000.

Recombinant human Cleaved Caspase-3 protein Full length protein datasheet ( ab52314). Abcam offers quality products including antibodies, assays and other 

Rat anti- mouse CD3  ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. 12 Feb 2019 abcam ab16502: RRID:AB_443394 anti phospho-NF-kB p65 abcam ab86299: abcam ab13847; RRID:AB_443014 anti NLRP3 antibody.

6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5

Knockout Tested Rabbit recombinant monoclonal Caspase-3 antibody [EPR18297]. Validated in WB, IP, IHC and tested in Mouse, Rat, Human. Cited in 43 publication(s).

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Company Abcam; Price Pricing Info Supplier Page View Company Product Page; Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.
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Hybridoma Bank) and anti-activated caspase-3 (1:500; Abcam, ab13847). primary anti-Rad51 (1:500; Abcam, ab109107) antibody added overnight.

ZERO BIAS - scores, article reviews, protocol conditions and more Anti Oxdj 1, supplied by Abcam, used in various techniques.
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Digital data. Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.

Cited in 136 publication(s). Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Abcam catalog: ab13847 total number of products: 19. domestic rabbit polyclonal reactivity: human, mouse, rat, Xenopus laevis application: western blot 产品报价：询价 更新时间：2013/6/20 13:23:01 产地：美国. 品牌：Abcam.

1652 DUAN et al: OBOVATOL INHIBITS TSCC VIA THE EGF-MEDIATED JAK-STAT PATHWAY protein activation, activated caspase 9/3 and apoptosis regu-lator Bax, and that it attenuated the expression of cyclin D1 in

Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation. Anti-Calreticulin Abcam ab22683 IF Anti-HMGB1 Abcam ab18256 IF Anti-LC3 Cell Signaling 2775s WB, IF Anti-ATG5 Abcam ab228668 WB Anti-ATG7 Abcam ab53255 WB Anti-p62 Abcam ab56416 WB Anti-caspase3a Abcam ab2302 WB Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB detection method by using a mouse and rabbit specific Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3 antibody, anti-active Caspase 3 antibody active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 body (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4°C overnight. The following day, after being washed with PBS buffer, the sections were incubated first with second-ary antibodies for 1 hour at room temperature and then with Then anti-VEGF antibody (1:200, Abcam), anti-NeuN (1:200, Millipore), anti-GFAP (1:2,000, Millipore) primary antibody were applied to the sections and incubated at 4°C overnight. .. Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature. 2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 specific antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting.

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam. 781 publications reference this Antibody. We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. If you have written or know of another publication, please do let us know!